Head of Department

Dr. Iwona Kowalska
tel. (+48) 81 4786 883 
e-mail: ikowalska@iung.pulawy.pl

Office: biochem@iung.pulawy.pl
tel. (+48) 81 4786 880

Department is located in the INCBR Center,
address:  Krancowa 8 Street, 24-100 Pulawy.

Research staff:

Prof. Wiesław Oleszek  
Prof. Anna Stochmal    
Dr. Iwona Kowalska  
Dr. Jerzy Żuchowski  
Dr. Mariusz Kowalczyk   
Dr. Justyna Krzyżanowska-Kowalczyk  
Dr. Dariusz Jędrejek  
Dr. Solomiia Kozachok   
MSc Barbara Moniuszko-Szajwaj 
MSc Eng. Łukasz Pecio  
MSc Eng. Sylwia Pawelec 
MSc Jarosław Mołdoch 
MSc Agata Soluch

The research profile of the Department

Offer

Equipment

NMR Laboratory – Nuclear Magnetic Resonance Spectroscopy 

The laboratory specializes in phytochemical analysis – organic compounds present in plants – specialized (secondary) metabolites, including phenolic acids, flavonoids and saponins. We have a set of experiments that effectively allow you to study the type of aglycon and the connection between sugars. We offer both qualitative and quantitative analyzes using the ERETIC2 method, but also professional assistance in interpreting results.

AVANCE III HD 500 MHz Bruker spectrometer

Department is equipped with Bruker’s spectrometer, with probe-heads for measurements in liquid samples (20-100°C):

Typical applications for this instrument are:

Contact:
MSc Eng. Łukasz Pecio
e-mail: lpecio@iung.pulawy.pl
phone: +48 81 47 86 882

MS Laboratory – mass spectrometry

Research interests of the Department are focused on analysis of organic compounds synthesized by plants, especially plant specific (secondary) metabolites. The identification as well as the determination of their quantitative (also trace) content in the tested material is performed of mass spectra in MS and MSn (fragmentation spectra) modes measurements.

Bruker Impact II HD Q-TOF mass spectrometer with auxiliary equipment

Spectrometer aids the determination of the elemental composition of the chemical compounds under investigation by precisely measuring the mass-to-charge ratio of their ions. The hybrid design based on a quadrupole analyzer combined with a time-of-flight analyzer also allows obtaining fragmentation spectra for compounds identification. The spectrometer is equipped with interchangeable ion sources to ionize a wide variety of chemicals by electrospray (ESI), atmospheric-pressure chemical ionization (APCI, GC-APCI), or atmospheric-pressure photoionization (APPI).

Comprehensive qualitative and quantitative analyses of complex mixtures, such as plant extracts, are possible due to to a combination of the spectrometer with the supporting equipment: ultra-efficient liquid chromatography systems (UHPLC, Dionex UltiMate 3000RS), gas chromatography (GC, Bruker Scion 456) and capillary electrophoresis (CE, Agilent 7100).

Typical applications for this instrument are:

Contact:
Dr. Mariusz Kowalczyk
e-mail: mkowalczyk@iung.pulawy.pl
phone: +48 81 47 86 886

ACQUITY UPLCTM ultra-high performance liquid chromatography (UHPLC) system

The ACQUITY UPLCTM system is equipped with a photodiode array detector (PDA; the wavelength range 190 – 500 nm), coupled with a triple quadrupole low-resolution mass spectrometer (TQ Detector, Waters). The mass spectrometer with an ESCi® ion source can be operated in the electrospray (ESI) or atmospheric pressure chemical ionisation (APCI) mode. System is suited for work with reversed-phase (RP) or HILIC columns.

Typical applications for this instrument are:

Contact:
Dr. Dariusz Jędrejek
e-mail: djedrejek@iung.pulawy.pl
phone: +48 81 47 86 886

High performance liquid chromatography (HPLC) system

The Department is equipped with liquid chromatography system types:

Typical applications for these instruments are:

Contact:
MSc Jarosław Mołdoch
e-mail: jmoldoch@iung.pulawy.pl
phone: +48 81 47 86 885

Sample preparation laboratory

The Department equipment enables to samples preparation with all caution at each step of the process for the sake of chemically unstable substances.

CHRIST’s Gamma 2-16 LSC freeze dryer

Freeze dryer enabling freeze drying of samples in a wide range of temperatures (-55°C) – (+50 °C) and pressures (<0.01 mbar):

The device allows sublimation removal of water (and some organic solvents, e.g. t-BuOH, DMSO) at reduced temperature and under reduced pressure, from frozen solutions and biological materials, e.g. extracts, suspensions, whole plants and fruit / vegetables. Due to the use of reduced temperature, it is possible to dry thermolabile samples.

Freeze drying is carried out on 5 shelves with a diameter of 20 cm and 3 shelves with a diameter of 37 cm.

Accelerated Solvent Extraction System Dionex ASE 200

Accelerated Solvent Extraction System Dionex ASE 200 allow to significantly reduce the extraction time and solvent consumption by up to 90%. The autosampler with a 24-site rotor and extraction cells volumes available in 1 – 33 mL enable a series of analyzes with a wide range of samples weight. The system can work with aqueous and organic solvents. Temperature and pressure control accelerates the kinetics of the extraction process. The solvent boiling prevention makes extraction safer, faster and more efficient than traditional techniques.

The Department is equipped with various sizes of vacuum evaporators with coolant liquid recirculation.

Heidolph semi-technical rotary evaporator (up to 20 liters) 

Rotary evaporator which enables the evaporation of solvents with a wide range of boiling points thanks to the chemically resistant KNF SBC LABOBASE 860 vacuum pump (60 L/min, 2 mbar. final pressure) and an efficient cooler powered by a Huber Unichiller UC025 circulator (2.5 kW at 15 °C).The device allows for quick evaporation of both organic solvents and water from plant extracts and other materials while maintaining a low temperature of the solution, which is very important when processing temperature-sensitive substances. Additionally, it is possible to evaporate solutions that tend to spatter and foam thanks to the additional expansion vessel.

Multivapor P – 12 Buchi 

Multivapor rising laboratory efficiency and throughput. It can work with many types of solvents through the use of many different operating parameters, such as the bath temperature or the shaking speed. The system of separate baths ensures safety in terms of cross-contamination of samples. The system is equipped with a V-710 Buchi vacuum pump with a capacity of 3.1 m3/h, guaranteeing an ultimate vacuum of ≤ 1.5 mbar and a 500W F-105 Buchi recirculation pump.