Department of Biotechnology and Plant Breeding (NBH)

Head of Department:

dr Grażyna Korbecka-Glinka
tel. 81 4786 942
mobile: 516 203 536
e-mail: Grazyna.Korbecka-Glinka@iung.pulawy.pl

Breeding new cultivars of hops and tobacco towards quality and resistance using biotechnological, cytogenetic and molecular methods.
Studies on the impact of biotic and abiotic factors on changes in gene expression;
Diagnostics of viral and fungal diseases as well as identification and assessment of the diversity of crop plant pathogens (mycological analysis, DAS-ELISA, PCR, real-time PCR, Sanger sequencing and NGS).
The use of tissue and suspension cultures in plant breeding and biosynthesis of natural compounds under in vitro conditions.
Collection, maintenance of viability and genetic purity, and evaluation of collection materials of the genera Nicotiana and Humulus.
Examination of the content of selected plant secondary metabolites (including tobacco alkaloids, essential oils, hop bitter acids, and flavonoids) using chromatographic, spectrophotometric, and flow analysis methods.
Studies on the viability and health status of seeds of various plant species (germination tests according to International Seed Testing Association methodology, seed mycological analysis, mycotoxin content).

Chemical analyses of secondary metabolites of hops and tobacco: hop bitter acids by HPLC and essential oils of hops and tobacco alkaloids by GC/MS.
Advisory services in hop and tobacco production: diseases and pests, cultivation and fertilization, integrated plant protection.
Sale of seeds of new tobacco varieties and hop seedlings free from viruses and latent viroid.
Sequencing and analysis of DNA fragments using the 3500 Genetic Analyzer capillary sequencer.
Verification of the cultivar identity of hop raw material using DNA microsatellite marker analysis.
Mycological analysis of seeds and species identification of the isolated fungi.
Seed germination tests according to the International Seed Testing Association methodology.

Chromatography systems: GC/MS, Agilent 7890A, Agilent 5975C.
Flow cytometer: Sysmex Partec CyFlow Cube 8.
Systems LC/MS: Agilent 1200, Agilent 6110.
Microscopes: Nikon Eclipse 80i with fluorescence module, Nikon C-DSD230 stereoscopic.
Thermocyclers: Rotor-Gene 6500 5-plex HRM (Corbett Research), QuantStudio 5 i Veriti Thermal Cycler (Thermo Fisher Scientific), T Personal (Biometra), C1000, S1000 i PTC Tempo 96 (Bio-Rad)
Sequencers: Illumina MiSeq, Life Technologies 3500 Genetic Analyzer.
UV/Vis spectrophotometer: Tecan Infinite 200 Pro, ND-2000 NanoDrop, Implen NanoPhotometer N60.
Bioanalyzer: Agilent Bioanalyzer 2100.
Sunrise Tecan microplate reader.
Freeze-dryer: Alpha 1-4 LD plus Christ.
Hamilton STARlet pipetting robot.
Automated nucleic acid extractor: Taiwan Advanced Nanotech Maelstrom 4810.
Snijders Labs MC 1750 climate chambers with adjustable spectral composition of light, temperature, humidity and CO₂ concentration.