Department of Biotechnology and Plant Breeding (NBH)

Manager:

dr Urszula Skomra
tel. 81 4786 943
mobile: 516 203 542
e-mail: Urszula.Skomra@iung.pulawy.pl

breeding new cultivars of hops and tobacco towards quality and resistance using traditional, biotechnological and cytogenetic methods;
applying tissue cultures in genetic and biochemical research;
research on crops and pathogens using molecular biology techniques;
diagnosis of diseases and identification of pathogens;
qualitative, immunological and adaptation tests on wild species and varieties collected in the hops and tobacco collection;
examination of the content of selected plant secondary metabolites using chromatographic and conductometric methods (tobacco alkaloids, essential oils, bitter acids and hop flavonoids).

Chemical analyses of secondary metabolites of hops and tobacco: hop gentian acids by HPLC and essential oils of hops and tobacco alkaloids by GC/MS.
Advisory service on hop and tobacco production: diseases and pests, soil cultivation and fertilization, integrated plant protection.
Sequencing and analysis of DNA fragments using the Genetic Analyzer 3500 capillary sequencer.
Verification of varietal identity of hop material using DNA microsatellite marker analysis.

Chromatography systems: GC/MS, Agilent 7890A, Agilent 5975C.
Flow cytometers: Beckmann Coulter Cell Lab Quanta, Sysmex Partec CyFlow Cube 8.
Systems LC/MS: Agilent 1200, Agilent 6110.
Microscopes: Nikon Eclipse 80i with fluorescence module, Nikon C-DSD230 stereoscopic.
Thermocyclers: Rotor-Gene 6500 5-plex HRM Corbett ReseTarch, T Personal Biometra, C1000i S1000 Bio-Rad, Veriti Thermal Cycler Life Technologies.
Sequencers: Illumina MiSeq (NGS), 3500 Genetic Analyzer Life Technologies.
UV/Vis spectrophotometer: tecan infinite 200 Pro, ND-2000 NanoDrop.
Bioanalyzer: Agilent Bioanalyzer 2100.
Sunrise Tecan microplate reader.
Freeze-dryer: Alpha 1-4 LD plus Christ.
Hamilton STARlet pipetting robot.
Ultrasonic Lab freezers U 725 Innova New Brunswick Scientific.
Snijders Labs MC 1750 climate chambers with adjustable spectral composition of light, temperature, humidity and CO₂ concentration.